mouse mono clonal antibodies against flag  (Proteintech)

Journal: Cell & Bioscience

Article Title: IL-27 signaling mediates skin inflammation in experimental psoriasis and atopic dermatitis

doi: 10.1186/s13578-025-01527-2

Figure Lengend Snippet: IL-27RA deficiency ameliorates IMQ-induced psoriasis-like skin inflammation in mice. A Experimental design for IMQ treatment in B-K. B-C Flow cytometry results of IL-27RA expression on T cells of skin-draining lymph nodes from IMQ-treated WT ( n = 4) and KO ( n = 3) mice. Representative flow cytometry plots (B) and ratio of IL-27RA + T cells to live cells (C) are shown. D Representative photographs of IMQ-treated WT and KO mice on day 5. E Quantification of the mPASI score. n = 5–7 mice per group. F Representative skin histology (H&E staining) on day 5. Scale bar = 100 μm. G Quantification of epidermal thickness. n = 5–7 mice per group. H Representative immunohistochemistry images of Ki67 protein in the lesional skin of IMQ-treated WT and KO mice on day 5. n = 4 mice per group. Scale bar = 50 μm. I Quantification of Ki67 + epidermal cells in the lesional skin of IMQ-treated WT and KO mice on day 5. n = 4 mice per group. J-K Representative immunofluorescence images of keratin 1 (J) and filaggrin (K) in the lesional skin of IMQ-treated WT and KO mice on day 5. n = 4 mice per group. Scale bar = 50 μm. DAPI stains the nuclei. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. p values were calculated via 2-tailed unpaired Student’s t test (C, G and I) or two-way ANOVA (E)

Article Snippet: After blocking with 3% bovine serum albumin (BSA) for one hour at room temperature (RT), the sections were incubated overnight at 4 °C with a rabbit anti-mouse TNF-α polyclonal antibody (1:500, Servicebio, Cat# GB115702-100) or a mouse anti-Ki67 mono clonal antibody (1:500, Servicebio, Cat# GB121141-100).

Techniques: Flow Cytometry, Expressing, Staining, Immunohistochemistry, Immunofluorescence

Journal: Cell & Bioscience

Article Title: IL-27 signaling mediates skin inflammation in experimental psoriasis and atopic dermatitis

doi: 10.1186/s13578-025-01527-2

Figure Lengend Snippet: IL-27RA deficiency reduced MC903-induced AD-like skin inflammation in mice. A Representative photographs of MC903-treated WT and KO mice on day 11. B Ear thickness (mean ± SEM) of MC903-treated WT ( n = 10) and KO ( n = 8) mice. C Representative skin histology (H&E staining) on day 11. Scale bar = 100 μm. D Quantification of epidermal thickness. n = 8–9 mice per group. E Representative immunohistochemistry images of Ki67 in the lesional skin of MC903-treated WT and KO mice on day 11. n = 4 mice per group. Scale bar = 50 μm. F Quantification of Ki67 + epidermal cells in the lesional skin of MC903-treated WT and KO mice on day 11. n = 4 mice per group. G-H Representative immunofluorescence images of keratin 1 (G) and filaggrin (H) in the lesional skin of MC903-treated WT and KO mice on day 11. n = 4 mice per group. Scale bar = 50 μm. DAPI stains the nuclei. ** p < 0.01, **** p < 0.0001. p values were calculated via two-way ANOVA (B) or 2-tailed unpaired Student’s t test (D and F)

Article Snippet: After blocking with 3% bovine serum albumin (BSA) for one hour at room temperature (RT), the sections were incubated overnight at 4 °C with a rabbit anti-mouse TNF-α polyclonal antibody (1:500, Servicebio, Cat# GB115702-100) or a mouse anti-Ki67 mono clonal antibody (1:500, Servicebio, Cat# GB121141-100).

Techniques: Staining, Immunohistochemistry, Immunofluorescence

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: CD147 mediates the adsorption of influenza A virus on the cell surface through direct interaction with HA

doi: 10.3389/fcimb.2025.1647283

Figure Lengend Snippet: Virus infection, adsorption and glycosylation modified form of CD147. (A) DF1 cells were infected with H5N6 (JX) virus (MOI 0.001), and the cells were harvested and analyzed the change of protein level via WB. (C) A549 cells were infected with H1N1 (PR8) virus (MOI 0.01), and the cells were harvested and analyzed the change of protein level via WB. (E) A549 cells were exposed to PD1530356h, 6h 12h and 24h with different concentrations, and infected with PR8(MOI 10) at 4°C for 1h, the cells were harvested and analyzed the change of protein level via WB. (G) MDCK cells were exposed to PD1530356h 24 hours, and infected with H9N2 (MOI 10) at 4°C for 1h, the cells were harvested and analyzed the change of protein level via WB. (B, D, F, H) Gapdh was used as the control, Image J(NIH) was used to quantitatively analyze the gray level of protein bands, and CD147/Gapdh indicated the relative level of CD147. All comparisons were tested by two-tailed Student’s t test, the solid line represents the difference of HG-CD147 in different infection periods, and the dotted line represents the difference of LG-CD147 in different infection periods, blue * represents the difference of LG-CD147 in the same infection period, red * represents the difference of HG-CD147 in the same infection period, and black * represents the difference of total protein of CD147. *, P, 0.05; **, P, 0.01; ***, P, 0.001; ****, P, 0.001.

Article Snippet: The following antibodies were used: anti-Flag mouse monoclonal antibody (Sigma, Germany, #F1804); anti-CD147 rabbit monoclonal antibody, anti-CD147 mouse poly-clonal antibody, anti-HA rabbit polyclonal antibody, and anti-GAPDH mouse mono-clonal antibody (Wuhan Sanying, #11989-1-AP, #66443-1-Ig, #51064-2-Ig, and #60004-1-Ig, respectively); anti-Strep mouse monoclonal antibody (AbClon, #AE066); and rabbit polyclonal antibodies against IAV proteins HA, NA, M1, M2, and NP (GeneTex, #GTX127357, #GTX125974, #GTX125928, #GTX640415, and #GTX125923, respectively).

Techniques: Virus, Infection, Adsorption, Glycoproteomics, Modification, Control, Two Tailed Test